Tripeptidyl peptidase IH in haemolysates and liver homogenates
نویسنده
چکیده
Rabbit antibodies against purified tripeptidyl peptidase II (TPP II) of human erythrocytes have been prepared and used in immunoblot analysis. Antibodies affinity-purified against the denatured 135000-Mr subunit of the human peptidase were shown to cross-react with purified rat liver TPP II, as well as a polypeptide ofMr 135000 in haemolysates and liver homogenates from several other species. TPP II activity in haemolysates from monkey, hog, horse and rabbit corresponded to the levels found in human erythrocytes, whereas this activity was absent or low in erythrocytes from calf and hen. On immunoblot analysis of the calf haemolysates, the 135000-Mr polypeptide could not be detected. In contrast, analysis of liver homogenates from these species revealed both TPP activity and immunoreactive polypeptides. Immunoreactive polypeptides with Mr about 105000 and 84000 of variable occurrence were also detected. In addition, extracts ofEscherichia coli showed no TPP II activity under the conditions of the standard assay, although polypeptides of Mr 135000 and 40000 were revealed on immunoblot analysis of this species.
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